St. Luke's eight (8) departments assemble traditional medical services into specialties, imparting dedicated medical expertise and support facilities to the areas it cover.

The individual departments are the leading choices for primary health services among Philippine hospitals. St. Luke's operates the Departments in Medicine, Nuclear Medicine, Obstetrics and Gynecology, Surgery, Otorhinolaryngology, Pediatrics, Anesthesiology, Physical Medicine and Rehabilitation, Emergency Medicine, Out-Patient Care, Legal Medicine and Jurisprudence, and Dentistry and Oral Surgery.

Each of these departments boasts of the most advanced equipment, unmatched by any local hospital, new or old. Further, its comprehensive array of services is supported with continuous training and research to improve the quality of its care.